Owensboro is a city in Daviess County, Kentucky, United States, of which it is also the county seat. It is the fourth-most populous city in the state. Owensboro is located on U.S. Route 60 and Interstate 165 about 107 miles (172 km) southwest of Louisville, and is the principal city of the Owensboro metropolitan area. The 2020 census had its population at 60,183. The metropolitan population was estimated at 116,506. The metropolitan area is the sixth largest in the state as of 2018, and the seventh largest population center in the state when including micropolitan areas.