Titusville is a city in and the county seat of Brevard County, Florida, United States. As of the 2020 census, the population of the city was 48,789, up from 43,761 at the 2010 census. Titusville is located along the Indian River, west of Merritt Island and Kennedy Space Center, and south-southwest of Canaveral National Seashore. It is a principal city of the Palm Bay–Melbourne–Titusville, Florida Metropolitan Statistical Area.