Alachua is the second-most populous city in Alachua County, Florida, and the third-largest in North Central Florida. According to the 2020 census, the city's population was 10,574, up from 9,059 at the 2010 census. It is part of the Gainesville, Florida Metropolitan Statistical Area. Alachua has one of the largest bio and life sciences sectors in Florida and is the site for the Santa Fe College Perry Center for Emerging Technologies.